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pcw cas9 hpgk puror plasmid  (Addgene inc)


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    Structured Review

    Addgene inc pcw cas9 hpgk puror plasmid
    Pcw Cas9 Hpgk Puror Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 346 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcw+cas9+hpgk+puror+plasmid/pCW-Cas9+(Plasmid+%2350661)/pmc13040219-189-14-16
    Average 96 stars, based on 346 article reviews
    pcw cas9 hpgk puror plasmid - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    CRISPR:

    Article Title: LINE-1 Locus Transcription Nucleates Oncogenic Chromatin Architecture
    Article Snippet: The inducible CRISPR inhibition construct was generated by sub-cloning the 3xFLAG-dCas9-KRAB fragment from the pLV-hUbC-KRAB-dCas9-2A-PuroR vector (Addgene, #71236) along with a P2A-TagBFP insert into the inducible pTRIPZ backbone harboring an EF1α-driven Tet-On 3G cassette fused to 2A-PuroR. .. The inducible CRISPR knockout construct was generated by subcloning 3xFLAG-Cas9 and IRES-TagBFP into the pCW-Cas9-hPGK-PuroR plasmid (Addgene, #50661). .. Inserts were generated by PCR with Q5 High-Fidelity DNA Polymerase (New England Biolabs, M0491L), and subcloning was done by NEBuilder HiFi DNA Assembly (New England Biolabs, E5520L). sgRNA vectors were generated by subcloning mCherry or ZsGreen fused to 2A-BSD (blasticidin resistance gene) downstream of the EF1α promoter in the LentiGuide-Puro vector replacing the PuroR cassette (Addgene, #52963).

    Knock-Out:

    Article Title: LINE-1 Locus Transcription Nucleates Oncogenic Chromatin Architecture
    Article Snippet: The inducible CRISPR inhibition construct was generated by sub-cloning the 3xFLAG-dCas9-KRAB fragment from the pLV-hUbC-KRAB-dCas9-2A-PuroR vector (Addgene, #71236) along with a P2A-TagBFP insert into the inducible pTRIPZ backbone harboring an EF1α-driven Tet-On 3G cassette fused to 2A-PuroR. .. The inducible CRISPR knockout construct was generated by subcloning 3xFLAG-Cas9 and IRES-TagBFP into the pCW-Cas9-hPGK-PuroR plasmid (Addgene, #50661). .. Inserts were generated by PCR with Q5 High-Fidelity DNA Polymerase (New England Biolabs, M0491L), and subcloning was done by NEBuilder HiFi DNA Assembly (New England Biolabs, E5520L). sgRNA vectors were generated by subcloning mCherry or ZsGreen fused to 2A-BSD (blasticidin resistance gene) downstream of the EF1α promoter in the LentiGuide-Puro vector replacing the PuroR cassette (Addgene, #52963).

    Construct:

    Article Title: LINE-1 Locus Transcription Nucleates Oncogenic Chromatin Architecture
    Article Snippet: The inducible CRISPR inhibition construct was generated by sub-cloning the 3xFLAG-dCas9-KRAB fragment from the pLV-hUbC-KRAB-dCas9-2A-PuroR vector (Addgene, #71236) along with a P2A-TagBFP insert into the inducible pTRIPZ backbone harboring an EF1α-driven Tet-On 3G cassette fused to 2A-PuroR. .. The inducible CRISPR knockout construct was generated by subcloning 3xFLAG-Cas9 and IRES-TagBFP into the pCW-Cas9-hPGK-PuroR plasmid (Addgene, #50661). .. Inserts were generated by PCR with Q5 High-Fidelity DNA Polymerase (New England Biolabs, M0491L), and subcloning was done by NEBuilder HiFi DNA Assembly (New England Biolabs, E5520L). sgRNA vectors were generated by subcloning mCherry or ZsGreen fused to 2A-BSD (blasticidin resistance gene) downstream of the EF1α promoter in the LentiGuide-Puro vector replacing the PuroR cassette (Addgene, #52963).

    Generated:

    Article Title: LINE-1 Locus Transcription Nucleates Oncogenic Chromatin Architecture
    Article Snippet: The inducible CRISPR inhibition construct was generated by sub-cloning the 3xFLAG-dCas9-KRAB fragment from the pLV-hUbC-KRAB-dCas9-2A-PuroR vector (Addgene, #71236) along with a P2A-TagBFP insert into the inducible pTRIPZ backbone harboring an EF1α-driven Tet-On 3G cassette fused to 2A-PuroR. .. The inducible CRISPR knockout construct was generated by subcloning 3xFLAG-Cas9 and IRES-TagBFP into the pCW-Cas9-hPGK-PuroR plasmid (Addgene, #50661). .. Inserts were generated by PCR with Q5 High-Fidelity DNA Polymerase (New England Biolabs, M0491L), and subcloning was done by NEBuilder HiFi DNA Assembly (New England Biolabs, E5520L). sgRNA vectors were generated by subcloning mCherry or ZsGreen fused to 2A-BSD (blasticidin resistance gene) downstream of the EF1α promoter in the LentiGuide-Puro vector replacing the PuroR cassette (Addgene, #52963).

    Subcloning:

    Article Title: LINE-1 Locus Transcription Nucleates Oncogenic Chromatin Architecture
    Article Snippet: The inducible CRISPR inhibition construct was generated by sub-cloning the 3xFLAG-dCas9-KRAB fragment from the pLV-hUbC-KRAB-dCas9-2A-PuroR vector (Addgene, #71236) along with a P2A-TagBFP insert into the inducible pTRIPZ backbone harboring an EF1α-driven Tet-On 3G cassette fused to 2A-PuroR. .. The inducible CRISPR knockout construct was generated by subcloning 3xFLAG-Cas9 and IRES-TagBFP into the pCW-Cas9-hPGK-PuroR plasmid (Addgene, #50661). .. Inserts were generated by PCR with Q5 High-Fidelity DNA Polymerase (New England Biolabs, M0491L), and subcloning was done by NEBuilder HiFi DNA Assembly (New England Biolabs, E5520L). sgRNA vectors were generated by subcloning mCherry or ZsGreen fused to 2A-BSD (blasticidin resistance gene) downstream of the EF1α promoter in the LentiGuide-Puro vector replacing the PuroR cassette (Addgene, #52963).

    Plasmid Preparation:

    Article Title: LINE-1 Locus Transcription Nucleates Oncogenic Chromatin Architecture
    Article Snippet: The inducible CRISPR inhibition construct was generated by sub-cloning the 3xFLAG-dCas9-KRAB fragment from the pLV-hUbC-KRAB-dCas9-2A-PuroR vector (Addgene, #71236) along with a P2A-TagBFP insert into the inducible pTRIPZ backbone harboring an EF1α-driven Tet-On 3G cassette fused to 2A-PuroR. .. The inducible CRISPR knockout construct was generated by subcloning 3xFLAG-Cas9 and IRES-TagBFP into the pCW-Cas9-hPGK-PuroR plasmid (Addgene, #50661). .. Inserts were generated by PCR with Q5 High-Fidelity DNA Polymerase (New England Biolabs, M0491L), and subcloning was done by NEBuilder HiFi DNA Assembly (New England Biolabs, E5520L). sgRNA vectors were generated by subcloning mCherry or ZsGreen fused to 2A-BSD (blasticidin resistance gene) downstream of the EF1α promoter in the LentiGuide-Puro vector replacing the PuroR cassette (Addgene, #52963).



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